AFC Meetings

AFC Summer 2013 meeting

AFC Summer 2013

Held at University College, Dublin

July 4th-5th 2013

Scientific Programme

Session 1: University College Dublin Symposium and guest speakers

Guest speaker
Prof Patrick Brennan, Colorado State University

Host preference across the Mycobacterium tuberculosis complex
Stephen Gordon, University College Dublin

Wildlife intervention strategies for control of bovine tuberculosis
Eamonn Gormley, School of Veterinary Medicine, UCD, Dublin

Session 2: Trinity College Dublin Symposium and guest speakers

Invited talk: TB vaccine efficacy trials:what can we learn and what’s next?
Helen McShane, University of Oxford

Joe Keane, Trinity College Dublin

Phylogenetic lineages of tuberculosis isolates associate with specific patient demographics in New Zealand
Ronan F O’Toole, Trinity College Dublin

The ancient evolution of tuberculosis as a zoonosis
David E Minnikin, University of Birmingham, Birmingham

Session 3: Offered papers

David McHugh, University College Dublin

Genetic Analyses of a Conserved Virulence Regulon in the Mycobacterium tuberculosis complex
Claire Healey, University College Dublin

The Synthesis and Biological Evaluation of Mycobacterial p-Hydroxybenzoic Acid Derivatives (p-HBADs)
Eoin Sacnlan, Trinity College Dublin

My-D88 adapter like (Mal) is required for effective macrophage responses To Mycobacterium tuberculosis
Cliona Ni Cheallaigh, Trinity College Dublin

Insights into the global metabolic changes in mycobacteria during nitrogen stress
Kerstin Williams, Imperial College London

Session 4: Offered papers

Biofilms and antibiotic tolerance: transcriptomic profiles of Mycobacterium smegmatis biofilms
S.A. Lethbridge, Public Health England, Porton Down, Salisbury

Genome-wide mapping of transcription start sites defines an extensive leaderless transcriptome in Mycobacterium tuberculosis
Teresa Cortes,National Institute for Medical Research, MRC, London

A transposon-mutagenesis library to study mechanisms of drug resistance in Mycobacterium tuberculosis
Joana Fonseca, University College London

Mannan core branching of lipo(arabino)mannan is required for initiation of mycobacterial granuloma formation in vivo
Arun K. Mishra, MRC National Institute of Medical Research, London

Mycobacterium leprae – A genome lost in time
Thomas A. Mendum, University of Surrey

AFC Winter 2013 meeting

AFC Winter 2013

Held at the Royal Free Hospital, London

January 11th 2013

Scientific Programme

Session 1

Human plasma miRNA and snoRNA molecules are significantly downregulated in tuberculosis-infected individuals in response to chemotherapy but HIV-1 co-infection spoils the picture
Isobella Honeyborne, Shirin Bokari, Silvana Christou, Clare Eckold, Alexander Pym, Timothy McHugh. UCL, London

Carbon fixation and a mixed diet for intracellular Mycobacterium tuberculosis
Dany J V Beste, Katharina Nöh, Sebastian Niedenführ, Tom A Mendum, Nathaniel D Hawkins, Jane L Ward, Michael H Beale, Wolfgang Wiechert and Johnjoe McFadden. Faculty of Health and Medical Sciences, Department of Microbial and Cellular Sciences, University of Surrey

Destruction of lung extracellular matrix by MMP-1 is critical in TB pathogenesis
Basim Al shammari, Takayuki Shiomi, Liku Tezera, Brian D Robertson, Jon S Friedland, Jeanine D’Armiento and Paul Elkington. Department of Infectious Diseases and Immunity, Imperial College London

Non-replicating Mycobacterium tuberculosis elicits a reduced pathogenicity profile and corresponding modifications to the cell wall
Bacon J, Alderwick LJ, Allnutt JA, Gabasova E, Watson R, Hatch KA, Clark SA, Marriott A, Rayner E, Tolley H, Pearson G, Hall G, Besra GS, Wernisch L, Williams A, Marsh PD. Health Protection Agency, Porton Down, Salisbury

Analysis of host gene induction following MVA85A
M. Matsumiya, Z. Lang, J. Meyer, S. Harris, A. Pathan, R. Rowland, A. Minassian, H. Fletcher, H. McShane. The Jenner Institute, University of Oxford

Session 2

MTS2048 a small RNA with a secret
Joanna Houghton, Olga Schubert, Douglas Young and Kristine Arnvig. Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London

Structure of M. tuberculosis DprE1, the target of BTZ compounds
Sarah M Batt, Talat Jabeen, Veemal Bhowruth, Peter A Lund, Luke J Alderwick, Klaus Fütterer, Gurdyal S Besra. School of Biosciences, University of Birmingham

Discovery of the anti-tubercular specific activity of ibuprofen and other 2-arylpropanoic acids using the HT-SPOTi whole-cell phenotypic assay
Juan David Guzman, Dimitrios Evangelopoulos, Antima Gupta, Kristian Birchall, Solomon Mwaigwisya, Simon Gibbons, Barbara Saxty, John Malkinson, Timothy D. McHugh and Sanjib Bhakta. Mycobacteria Research Laboratory, Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, London

Selective ESAT6-dependent necrosis of phosphatidylserine externalised neutrophils
Robert J Francis, Rachel E Butler, Graham Stewart. Department of Microbial and Cellular Sciences, Faculty of Health and Medical Sciences, University of Surrey

Ethnic variation in inflammatory profile in tuberculosis
Adrian R. Martineau, Anna K. Coussens, Robert J. Wilkinson, Vladyslav Nikolayevskyy, Francis A. Drobniewski, Paul T. Elkington, Graham H. Bothamley, Robert N. Davidson, Christopher J. Griffiths. Queen Mary University of London, Barts and The London School of Medicine and Dentistry, Blizard Institute, London E1 2AB, Division of Mycobacterial Research, MRC National Institute for Medical Research, London

AFC Summer 2012 meeting

AFC Summer 2012

Held at the Brighton and Sussex Medical School, University of Sussex

July 5th-6th 2012

Scientific Programme

Session 1: Brighton and Sussex Medical School and University ofSussex Symposium

Genetic susceptibility to M.tuberculosis
Melanie Newport

Interactions between a novel transcriptional activator and RNA polymerase
Mark Paget

Flow-cytometry in the diagnosis of active TB
Florian Kern

DNA repair in mycobacteria
Aidan Doherty

The effect of growth rate on the efficacy of isoniazid against Mycobacterium tuberculosis
Alice Marriott, Jon Allnutt, Kim Hatch, Catherine Arnold, Tim McHugh, Philip Marsh, Joanna Bacon, HPA Microbiology Services, Porton Down, Salisbury

Session 2: Guest speaker

Host-pathogen interactions in TB
Olivier Neyrolles. CNRS, Toulouse

Session 3: Offered papers

Evaluating BCG and novel TB vaccine candidates with a human BCG challenge model
Joel Meyer, S Harris, I Satti, A Minassian, H Fletcher, H McShane, Jenner Institute, Oxford University

Does biofilm growth of Mycobacterium tuberculosis replicate features of the phenotypes observed in tuberculous sputum?
Abdulwhab Binjomah, Andrew Bell, Su-Min Lee, Galina Mukamolova, Natalie Garton and Mike Barer, Department of Infection Immunity and Inflammation, University of Leicester

The role of peptidoglycan glycolylation in mycobacterial dormancy and resuscitation
Jessica Loraine, Obolbek Turapov, Jacob Biboy, Waldemar VollmerandGalina Mukamolova, Infection, Immunity, Inflammation Department and Chemistry Department, University of Leicester

Transcriptional profiling of disease-induced host responses in bovine tuberculosis
Elihu Aranday-Cortes, Philip J. Hogarth, Daryan A. Kaveh, Adam O. Whelan, Bernardo Villarreal-Ramos, Ajit Lalvani, H. Martin Vordermeier, Animal Heath & Veterinary Laboratories Agency, Surrey

Measuring phosphorylation of the metabolic regulator GarA in living mycobacteria
Barbara Rieck, A. R. Bottrill, H M O’Hare, Department of Infection, Immunity, and Inflammation, University of Leicester

Session 3: Offered papers

Genome-wide mapping of the GlnR regulon in Mycobacterium smegmatis
Victoria Jenkins, Brian Robertson and Kerstin Williams, MRC CMBI, Flowers Building, Imperial College London, South Kensington, London

Identification of novel Mt-GuaB2 inhibitor series active against M. tuberculosis
Veeraraghavan Usha, Judith V. Hobrath, Sudagar S. Gurcha, Robert C. Reynoldsand Gurdyal S. Besra, School of Biosciences, University of Birmingham, Edgbaston, Birmingham

ATP-dependent Mur ligases in the biogenesis of cell wall peptidoglycan in Mycobacterium tuberculosis: novel targets for anti-TB drug discovery
Tulika Munshi, Antima Gupta, Dimitrios Evangelopolous, Juan David Guzman, Nicholas H. Keep, Simon Gibbons and Sanjib Bhakta, Mycobacteria Research Laboratory, Department of Biological Sciences, Institute of Structural & Molecular Biology, Birkbeck, University of London

Impaired host immune system favours tuberculosis induced by Mycobacterium africanum but not Mycobacterium tuberculosis
Leopold. D. Tientcheu, J. Sutherland, B. de Jong, S. Smith, H. M. Dockrell, M. O. Ota, Vaccinology Theme, Medical Research Council Unit The Gambia, and Department of Immunology and Infection, Faculty of Infectious and Tropical Disease, London School of Hygiene & Tropical Medicine

AFC Winter 2012 meeting

AFC Winter 2012

Held at Imperial College, London

January 6th 2012

Scientific Programme

Session 1

Independent impact of tuberculosis disease stage and HIV co-infection on Mycobacterium tuberculosis (MTB)-specific cellular immunity
Katrina M Pollock, Montamat-Sicotte D, Cooke G, Kapembwa M, Kon O M, Taylor G P and Lalvani A. Tuberculosis Research Unit, Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London

Mycobacterial Growth Indicator Tube (MGIT) assay as a correlate of protection against TB
Leanne Marsay, R. Tanner, H. Poyntz, K. Griffiths, E. Stylianou, M. Kostov, H. Fletcher, S. Sharpe, H. McShane. The Jenner Institute, University of Oxford, Oxford, UK

Increased diagnostic yield of sputum samples for Mycobacterium tuberculosis by loop mediated amplification (LAMP) compared to sputum microscopy in Kerbala, Iraq
Mohanad Ahmed, Hassan Abood, Natalie Garton and Mike Barer.West Euphrates Research Unit, Kerbala University, Kerbala, Iraq

Evaluation of the influence of the route of administration in the immunogenicity of Ad5-Ag85A as booster in BCG vaccinated cattle
Adam Whelan, Pinar Court, Glyn Hewinson, Xhou Zing, Derek Clifford, Martin Vordermeier, Bernardo Villarreal-Ramos. TB Research Group, Animal Health and Veterinary Laboratories Agency, Surrey

A TetR-type regulator represses the production of oleic acid in mycobacteria
Mahmudul Hasan Sikder, Mike Withers, Ricardo Balhana, Neil Stoker and Sharon Kendall. Department of Pathology and Infectious Diseases, The Royal Veterinary College, London

Session 2

Vitamin D accelerates resolution of inflammatory responses during tuberculosis treatment
Anna KCoussens, Hanifa Y, Nikolayevskyy V, Elkington PT, Islam K, Timms PM, Venton TR, Bothamley GH, Packe GE, Darmalingam M, Davidson RN, Milburn HJ, Baker LV, Barker RD, Mein CA, Bhaw-Rosun L, Nuamah R, Young DB, Drobniewski FA, Griffiths CJ, Wilkinson RJ, Martineau AR. Division of Mycobacterial Research, MRC National Institute of Medical Research, London

Expression of the two ClpP protease subunits in Mycobacterium tuberculosis
Yoann Personne, Amanda C. Brown, Tanya Parish. Barts and London School of Medicine and Dentistry, Queen Mary University of London

Phthiocerol dimycocerosate waxes are potential biomarkers for the rapid diagnosis of tuberculosis in sputum
David E. Minnikin, Ruth McNerney,Denise M. O’ Sullivan, Simona C. Nicoara, Elizabeth L. Corbett, Reggie Mutetwa, Stanley Mungofa, Oona Y-C. Lee, Max W. Bardwell, Geraint H. Morgan. School of Biosciences, University of Birmingham

Systems pharmacology approach to antimycobacterial drug discovery. Luiz Pedro S. de Carvalho and Douglas Young. Division of Mycobacterial Research, MRC National Institute for Medical Research, Mill Hill, London

Serodiagnosis of tuberculosis: due to change track
Juraj Ivanyi. Guy’s Campus of Kings College London

AFC Summer 2011 meeting

AFC Summer 2011

Held at the University of Surrey

July 8th 2011

Scientific Programme

Session 1: Surrey University

Interrogating transcriptomic data with a genome-scale model of metabolism: what does M. tuberculosis eat in vivo?
Bhushan Bonde, Dany Beste, Emma Laing, Andrzej M. Kierzek, Johnjoe McFadden

Cell death fates in Mycobacteruim tuberculosis infected             macrophages are influenced by multiplicity of infection, virulence, and strain characteristics
Rachel E. Butler, Priscille Brodin, Jichan Jang, Mi-Seon Jang, Hilde Smith, Jelle Thole, Brigitte Gicquel, Olivier Neyrolles and Graham R Stewart

Session 2

Exploring the therapeutic potential of a novel human IgA antibody against tuberculosis
Rajko Reljic, Sucharitha Balu, Jenny Woof and Juraj Ivanyi. St George’s, University of London

Genome Scale Modelling of Mycobacterium bovis and Mycobacterium bovis Bacille Calmette-Guérin
Emma Lofthouse, Paul Wheeler, Andrzej Kierzek, Dany Beste, Tom Mendum and Johnjoe McFadden. Tuberculosis Research Group, Animal Health and Veterinary Laboratories Agency,Weybridge

An evaluation of the safety and immunogenicity of MVA85A delivered by aerosol to the lungs of macaques
A.D. White, K. Gooch, L. Sibley, M.J. Dennis, G. Betts, A. Reyes-Sandoval, A. Williams, P.D. Marsh, H. McShane, S.A. Sharpe. Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury

Broad cytokine/chemokine response in peripheral blood stimulated with heparin-binding haemagglutinin following BCG vaccination of UK infants
Steven G. Smith, Sophie Lecher, Rose Blitz, Camille Locht, Hazel M. Dockrell. Department of Immunology and Infection, London School of Hygiene & Tropical Medicine, London

The use of low temperature incubation to screen for whole cell activity of drugs against persisters
D.Coleman, D.A.Mitchison, S Waddell, St Georges, University of London

Session 3

Mycobacterium leprae upregulates human beta defensins in cutaneous leprosy Type 1 Reactions
Anna L. Cogen, Steven L. Walker, Chrissy Roberts, Sushmita Dhakal, Rachel A. Hawksworth, Kishori Mahat , Diana N.J. Lockwood. Faculty of Infectious Tropical Diseases, Department of Clinical Research, London School of Hygiene and Tropical Medicine, London

Mycobacterium tuberculosis lineage influences innate immune response and is associated with distinct cell envelope lipids
Nitya Krishnan, Wladimir Malaga, Maxine Caws, Douglas.B.Young, Brian D. Robertson, Christophe Guilhot, Guy.E.Thwaites. Centre for Molecular Microbiology and Infection, Imperial College, London

The C-Terminal Domain of the Ethambutol target Mt-EmbC is a Lectin-like Carbohydrate Binding Module
Luke J. Alderwick, Georgina S. Lloyd, Hemza Ghadbane, John W. May, Apoorva Bhatt, Lothar Eggeling, Klaus Fütterer, Gurdyal S. Besra. School of Biosciences, University of Birmingham

A distinct profile of cytokine production correlates with and predicts BCG induced protection in a Mycobacterium bovis murine challenge model
Daryan A. Kaveh, Elihu Aranday-Cortes and Philip J. Hogarth, TB Research Group, Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey

Insights into immigrant screening for tuberculosis from evaluation of practice and a multi-centre cohort study of cost-effectiveness
Manish Pareek, John P. Watson, L Peter Ormerod, Onn Min Kon, Gerrit Woltmann, Peter J White, Ibrahim Abubakar, Geoffrey P Garnett, Ajit Lalvani, Tuberculosis Research Unit, National Heart and Lung Institute, Imperial College London, UK & Department of Infectious Disease Epidemiology, Imperial College London, UK

AFC Winter 2011 meeting

AFC Winter 2011

Held at St George’s University of London

January 7th 2011

Scientific Programme

Session 1

Tandem duplications of large genomic islands in Mycobacterium avium subspecies paratuberculosis associated with human strains isolated from patients with Crohn’s disease
Tim Bull, Richard Linedale, Wojtek Michalski, James Wynne. Department of Cellular and Molecular Medicine, Division of Clinical Sciences, St George’s, University of London

Functional analysis of the serine-threonine protein kinase, PknF, and its substrate the ABC transporter Rv1747, in Mycobacterium tuberculosis
Vicky L. Spivey, Virginie Molle, Rachael H. Whalan, Angela Rodgers, Apoorva Bhatt, Lasse Stach, Elizabeth M. Hirst, Gurdyal S. Besra, K. Barry Walker, Stephen J. Smerdon, Roger S. Buxton. MRC National Institute for Medical Research

Identification of biomarkers of treatment response in blood by transcriptomic analysis
Jackie Cliff, Ji-Sook Lee, Nicholas Constantinou, Jang-Eun Cho, Taane Clark, Katharina Ronacher, Paul van Helden, Gerhard Walzl, Hazel Dockrell. Department of Immunology and Infection, London School of Hygiene & Tropical Medicine

Gene products HsaD and HsaC in cholesterol metabolism of Mycobacterium tuberculosis
M. Abdul Aziz, N. Lack, R. Ballet, V. Cheng, N. Laurieri, S. Keany, A. Ryan, & E. Sim. Department of Pharmacology, University of Oxford

Lung heparin targeted vaccine delivery of mycobacterial antigens
Elena Stylianou, Ilaria Pepponi, Craig van Dolleweerd, Julian Ma and Rajko Reljic Division of Clinical Sciences, St George’s, University of London

Session 2

Immunological responses following BCG vaccination and Mycobacterium bovis challenge in the Eurasian badger
Roland Ashford, Sandrine Lesellier, Si Palmer, Sonya Gowtage, Deanna Dalley, Dipesh Dave, Laura Chamberlain, Benjamin Grundy, R.Glyn Hewinson, Brian Catchpole, Frank E. Aldwell, and Mark Chambers. Veterinary Laboratories Agency

Mycobacterium tuberculosis acg in the hspX gene cassette is required for growth and virulence in vivo
Yanmin Hu and Anthony R. M. Coates. Centre of Infection and Immunity, Division of Clinical Sciences, St George’s University of London

Identification of novel non-tuberculous mycobacterial antigens
A Checkley, D Wyllie, I Satti, T Scriba, H Fletcher, A Hill, W Hanekom, H McShane. Jenner Vaccine Institute, Oxford University

Analysis of M. tuberculosis strains from the North London isoniazid resistant outbreak shows independent evolution of drug resistance
R.J. Shorten, A. McGregor, S.H. Gillespie, C. Jenkins, S. Platt and T.D. McHugh. Department of Medical Microbiology, Royal Free Hospital, London & Centre for Clinical Microbiology, University College London

Differential small RNA expression across Mycobacterium bovis field strains
P. Golby, J. Nunez, J. Hinds, A. Witney, S. Bentley, G. Hewinson and S. Gordon. Veterinary Laboratories Agency

AFC Summer 2010 meeting

AFC Summer 2010

Held at the Veterinary Laboratories Agency, Weybridge

July 2nd 2010

Scientific Programme

Session 1

Mycobacterium bovis Mini-symposium
Glyn Hewinson (VLA).

Bovine Tuberculosis (bTB) is the most important animal health problem facing the country today. This mini symposium will outline the scientific advances made in understanding the spread of bTB and in developing new tools for its control. A question and answer session will allow members to discuss the current scientific evidence base and what can be concluded from it.

Session 2

Mycobacterium bovis-BCG vaccination induces specific transcriptome biosignatures in mice predicting vaccination success
Elihu Aranday-Cortes, Daryan Kaveh, Javier Nunez-Garcia, Philip J Hogarth, H. Martin Vordermeier Veterinary Laboratories Agency

Towards replicating the transcriptome of Mycobacterium tuberculosis in sputum by exposure to different in vitro conditions
Su-Min Lee, Natalie Garton and Mike Barer. University of Leicester

Mycobacterium tuberculosis RD1 is a requisite for virulence-associated cellular recruitment
Suzie Hingley-Wilson and Ajit Lalvani. Wright Fleming Institute of Infection & Immunity, Imperial College London

Session 3

Mycobacterium tuberculosis SigG does not control expression of DNA repair genes in response to DNA damage
Katherine L. Smollett, Lisa F. Dawson and Elaine O. Davis. MRC National Institute for Medical Research, London

Matrix metalloproteinase-1 causes tissue destruction in tuberculosis
Paul Elkington, Takayuki Shiomi, Ronan Breen, Robert K Nuttall, Cesar Augusto Ugarte-Gil, Naomi Walker, Luísa Saraiva, Bernadette Pedersen, Francesco Mauri, Marc Lipman, Dylan R Edwards, Brian D Robertson, Jeanine D’Armiento, & Jon S Friedland. Department of Infectious Diseases and Immunity, Imperial College London

Rapid and efficient construction of mycobacterial mutagenesis vectors using ligation-independent cloning
Ricardo J. C. Balhana, Neil G. Stoker, Sharon L. Kendall. The Royal Veterinary College, London.

Enumeration of polyfunctional antigen-specific T cells by fluorescence-linked-immunospot identifies immune signatures that correlate with distinct stages of tuberculosis infection
Rosalyn Casey, Deena Blumenkrantz, Kerry Millington, Damien Montamat-Sicotte, Onn Min Kon, Melissa Wickremasinghe, Samuel Bremang, Murphy Magtoto, Saranya Sridhar, David Connell & Ajit Lalvani. National Heart and Lung Institute, Imperial College London

New tuberculosis drugs targeted to the essential kinase, PknB
Kathryn E. A. Lougheed, Simon Osborne, Tim Chapman, Barbara Saxty, David Whalley, Tim Nott, Steve Smerdon, Dony Patel, Jasveen Chugh, Catherine Kettleborough, Justin Bryans, Debbie Taylor, Roger S. Buxton. MRC National Institute for Medical Research Mill Hill, London.

Expression and localisation of MPM70 during infection: evidence for a post-phagosomal role in pathogenesis
Alistair F. Ray, Kornelis R. Straatman, Lorna C. Waters, Phillip S. Renshaw, Kirsty L. Lightbody and Mark D. Carr. University of Leicester

AFC Winter 2010 meeting

AFC Winter 2010

Held at the London School of Hygiene & Tropical Medicine

January 8th 2010

Scientific Programme

Session 1

Pea Production by mycobacteria
Ruth McNerney and Claire Turner. Department of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine.

The Mycobacterium tuberculosis HigA antitoxin does not directly regulate any other genes in addition to its own operon
Amanda S. Fivian-Hughes and Elaine O. Davis. Division of Mycobacterial Research, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

The regulation of intracellular iron in the BCG-vaccinated host response to M. tuberculosis infection as determined by microarray
Ruth Thom, JA Tree, SO Clark, MJ Elmore, A Williams, PD Marsh. Centre for Emergency Preparedness and Response, Health Protection Agency, Porton Down, Salisbury, Wiltshire, SP4 OJG, UK

A salicylate-inducible promoter of Mycobacterium tuberculosis
Dorothee Schuessler, Amanda Brown and Tanya Parish. Queen Mary University of London, Barts and the London School of Medicine and Dentistry. CIID BICM 4, Newark Street London E1 2AT

Investigation of the stability of mycobacterial ribosomes during active growth and stasis
Andrej Trauner and Huw D. Williams. Department of Life Sciences, Imperial College, London

Session 2

Tribute to Chris Collins
given by Malcolm Yates

Human Natural Killer cell activity in response to M. bovis BCG versus M. tuberculosis H37Rv
Damien Portevin & Douglas Young. NIMR, London, UK

The Universal Stress Protein Rv1636 from Mycobacterium tuberculosis is a cation independent nucleotide triphosphatase
Dominic Bradley, Sergio Leiva, Simon Pennell, Ian A. Taylor, Stephen J. Smerdon, Huw D. Williams. Department of Life Sciences, Faculty of Natural Sciences, Imperial College, London

Granulocytes are necessary for the detection of mycobacteria-specific CD8+ T-cells responses in healthy individuals
Iman N. Satti, Natalie E.R. Beveridge, Helen A. Fletcher, Ansar A. Pathan, Thomas J. Scriba, Angela Minassian, Clare R. Sander, Willem A. Hanekom,Adrian V.S. Hill and Helen McShane. Jenner Institute, Old Road Campus Research Building, Nuffield Department of Medicine, Oxford University, Churchill Hospital, Old Road, Oxford, UK, OX3 7D

Optimisation of bioluminescent reporter constructs for in vivo imaging of mycobacteria
Nuria Andreu, Andrea Zelmer, Taryn Fletcher, Paul T. Elkington, Siouxsie Wiles and Brian D. Robertson. Department of Microbiology, Imperial College London, Flowers Building, Exhibition Road, London, SW7 2AZ, UK

AFC Summer 2009 meeting

AFC Summer 2009

Held at the Moredun Research Institute, Edinburgh

July 2nd-3rd 2009

Scientific Programme

Session 1: Research at the Moredun

Johne’s disease: an overview of research at Moredun
Karen Stevenson, Valerie Hughes, Craig A Watkins, Linda May, Susan Denham, Ian Heron and Joyce McLuckie:

Corynebacterium pseudotuberculosis – from genome to prevention
Florence E Pethick, Alex Lainson, Michael C Fontaine

Session 2

Phenotype microarray platform: Potential and pitfalls for use with pathogenic mycobacteria
Bhagwati Uphadyay and Paul R Wheeler. Veterinary Laboratories Agency, Weybridge

Fast growing, non-infectious and intra-cellularly surviving drug-resistant Mycobacterium aurum: A model for high-throughput anti-TB drug screening
Antima Gupta, Sanjib Bhakta, Subir Kundu, Manish Gupta, Brahm S. Srivastava, and Ranjana Srivastava. Central Drug Research Institute, India & Birkbeck College, University of London.

Kinetic Profiling of Immune responses to PE/PPE proteins in M. bovis-infected Cattle
H.M. Vordermeier, R.G. Hewinson, D.B. Young, S.L. Sampson. VLA Weybridge& Imperial College London

Session 3

Life and death in the mycobacterial phagosome
Rachel Shrimpton, Priscille Brodin, Olivier Neyrolles, Graham Stewart. Division of Microbial Sciences, University of Surrey, UK

Identification of 2-aminothiazole—4-carboxylate derivatives active against Mycobacterium tuberculosis H37Rv and the ketoacyl-ACP synthase mtFabH
Qosay Al-Balas, Nahoum G. Anthony, Bilal Al-Jaidi, Amani Alnimir, Grainne Abbott, Alistair K. Brown, Rebecca C. Taylor, Gurdyal. S. Besra, Timothy D. McHugh, Stephen H. Gillespie, Blair F. Johnston, Simon P. Mackay, Geoffrey D. Coxon. University of Strathclyde, Glasgow.

BCG: surrogate for a human TB challenge?
Minassian, A, Tchilian, E, Poulton, I, Fletcher, H, Andrews, L,   Poyntz, H, Ronan, E,   Hill, A, & McShane, H. The Jenner Institute, Oxford University

Acute and persistent Mycobacterium tuberculosis infections depend on the thiol peroxidase Tpx
Yanmin Hu and Anthony R. M. Coates. St George’s University of London.


Genes and proteins of the nat/hsaD gene cluster and their role in cholesterol metabolism
Nathan Lack, Dimitrios Evangelopoulos, Sanjib Bhakta, Lindsay Eltis, Areej Abuhammad, Elizabeth Fullam, Edward Lowe and Edith Sim. University of Oxford.

Session 4

Structure and functional characterisation of Mycobacterium tuberculosis diaminopimelic acid epimerase (MtDapF)
Veeraraghavan Usha, Lynn G. Dover, Klaus Fütterer, David L. Roper, Adrian J. Lloyd and Gurdyal S. Besra. University of Birmingham

The helicase UvrD2 is essential in Mycobacterium tuberculosis
Alan Williams, Burkhard Springer, Erik C. Böttger, and Elaine O. Davis. NIMR, Mill Hill, London

Dissection of regenerating T cell responses against tuberculosis in HIV infected adults with latent tuberculosis
Katalin A. Wilkinson, Ronnett Seldon, Graeme Meintjes, Molebogeng X. Rangaka, Willem A. Hanekom, Gary Maartens, Robert J. Wilkinson. University of Cape Town, South Africa

Live cell imaging to study growth and cell division in mycobacteria
Graham Joyce, Kerstin J. Williams, and Brian D. Robertson. Imperial College London

AFC Winter 2009 meeting

AFC Winter 2009

Held at the NIMR, Mill Hill

January 9th 2009

Scientific Programme

Session 1

Identification of small RNAs in Mycobacterium tuberculosis
Kristine B. Arnvig and Douglas B. Young. NIMR, London

Identification of TB contacts with latent TB infection in a population with a high HIV and TB burden
Kerry A Millington,Junior Mutsvangwa, Kathryn Chaka, Tafadzwa Mavhudzi, Yin-Bun Cheung, Peter R Mason, , Anthony E Butterworth, Elizabeth L Corbett and Ajit Lalvani. Imperial College, London

Characterisation of a putative dihydrofolate reductase from Mycobacterium smegmatis
Dimitrios Evangelopoulos, Nathan Lack, Giles Robertson, Tina Daviter, Nicholas H. Keep, Edith Simand Sanjib Bhakta. Birkbeck College, University of London

Changes in T cell population dynamics in 3 species: Dynamics of white cell populations in M. tuberculosis infection in rhesus macaques
K Gooch, S Sharpe, A Williams, M Dennis, P D Marsh. Health Protection Agency, CEPR, Porton Down

Detection and molecular characterization of 9000-year old Mycobacterium tuberculosis from a Neolithic settlement in the Eastern Mediterranean
Oona Lee, David Minnikin, Gurdyal Besra, Israel Hershkovitz, Vered Eshed, Ehud Galili, Charles Greenblatt, Eshetu Lemma, Gila Kahila Bar-Gal, Mark Spigelman, Helen Donoghue. University of Birmingham

Session 2

Inhibition of phago-lysosome fusion by Mycobacterium bovis BCG induces a Niemann-Pick type C1-like phenotype
Emyr Lloyd-Evans, Nathan Lack, Edith Sim and Frances M. Platt. University of Oxford

KstR2: Identification of a second TetR-type regulator involved in cholesterol catabolism in mycobacteria
Sharon Kendall, Annemieke ten Bokum, Phillipa Burgess, Mike Withers, Iria Uhia Castro, Nwaamaka Owoh, Nadia Mokhtar and Neil Stoker. The Royal Veterinary College, London

Intranasal vaccination with an ESAT-6 expressing attenuated influenza vaccine
A. Egorov. AVIR Green Hills Biotechnology, Vienna

BCG vaccination induces polyfunctional CD4+ T cells in the spleen which correlate with active immunity to a Mycobacterium bovis challenge
Philip J Hogarth. Veterinary Laboratories Agency, Weybridge.

The Burden of Mycobacterial Disease in Ethiopian Cattle
Stefan Berg, Rebuma Firdessa, Endalamaw Gadisa, Meseret Habtamu, Araya Mengistu, Lawrence Yamuah, Gobena Ameni, Brian D. Robertson, Douglas B. Young, Howard Engers, Noel H. Smith, Martin Vordermeier, R. Glyn Hewinson, Abraham Aseffa, and Stephen V. Gordon. Veterinary Laboratories Agency, Weybridge